What is the use of Erlenmeyer flask?

MixingErlenmeyer flask / Uses

Erlenmeyer flasks are used to contain liquids and for mixing, heating, cooling, incubation, filtration, storage, and other liquid-handling processes. Their slanted sides and narrow necks allow the contents to be mixed by swirling without the risk of spills, which is useful for titrations and for boiling liquids.

Why are Erlenmeyer flasks shaped that way?

Erlenmeyer Flask The flask has a conical base that almost extends into a small, cylindrical neck. This shape allows the flask to be sealed with a bung for heating purposes, while also allowing a researcher the freedom to shake or stir the flask without spilling liquid.

Can you reuse t75 flasks?

Yes, you can reuse flasks but you must do proper cleaning and sterilization. just autoclaving at appropriate temperature is enough subject to the health of culture vessels.

Can Erlenmeyer flasks be autoclaved?

Nonpyrogenic and RNase-/DNase-free BSE/TSE compliant accessories Erlenmeyer flasks are autoclavable once at 121°C, 15psi for 20 minutes. Repeated autoclaving weakens the flask and is not recommended.

How do Erlenmeyer flasks differ from beakers?

Erlenmeyer flasks have a narrow neck over a conical base, while beakers are basically large open-mouthed glass jars with a lip and spout for pouring. Graduated cylinders are tall cylinders with a spout to pour liquids; they have hash marks on the side to measure the volume of their contents.

What is the purpose of a watch glass?

A watch glass is a round, concave glass dish used for evaporation in chemistry. It can also be employed for weighing solids and as a lid for flasks and beakers.

Why are Erlenmeyer flasks used instead of beakers when heating reaction mixtures?

Erlenmeyer flasks are great for mixing chemicals since they have a wide base and can be swirled around without spillage. There is also the added advantage of the Erlenmeyer filter flasks’ ability to be stoppered using rubber bungs. This can’t be done to a beaker.

Why do we sterilize chemicals and glassware for tissue culture?

To maintain an aseptic environment, all culture vessels, media and instruments used in handling tissue, as well as the explant itself is should be surface sterilized. Plant material can be surface sterilized by variety of chemicals.

Can we autoclave 96 well plate?

96 well plates are not autoclavable and they will definitely melt.

How do you sterilize a culture flask?

Autoclave flasks that were used for growing microbial cultures on liquid cycle for 1 hour. Dump the sterilized contents of the flasks down the drain, rinse each flask a few times with DI water. Put flasks into the dishwasher (WEL: 2nd floor) and wash using cycle 7. This takes about 45 minutes.

What is the difference between flasks and beakers?

Flasks are notable for their unique shape: a rounded vessel and a cylindrical neck. The main differing characteristic between a flask and a beaker is that beakers have straight sides, rather than slanted sides like a flask. Beakers are mainly for measuring and transporting liquids from one site to the next.

Why is it called a watch glass?

Watch glasses are named so because they are similar to the glass used for the front of old-fashioned pocket watches. In reference to this, large watch glasses are occasionally known as clock glasses.

Why is a T-flask used for cell culture?

The T-flask is the most widely used vessel for routine maintenance of cell cultures. Its secure screw cap offers best protection of valuable cells. However, compared to dishes and plates, the T-flask provides only limited access to the cells due to its narrow neck which makes handling with pipettes and cell scrapers inconvenient.

What is the best Culture Media for insect cell culture?

Cells can be maintained at room temperature on the bench top or in a drawer, however, a 27°C controlled environment is recommended. Use media specifically formulated for insect cell growth. Use a surfactant to decrease shearing. 0.1\% Invitrogen Pluronic F-68 is recommended for spinner insect cultures.

What happens when cells reach confluency in a culture flask?

When they reach confluency, cells in suspension clump together and the medium appears turbid when the culture flask is swirled. The maximum recommended cell density before passaging varies with cell lines; refer to the cell-specific product insert or manual for details. Cells Grown in Shaker Flasks

What are the steps involved in subculturing insect cells?

Regular subculturing requires the removal of cell suspension and the addition of medium sufficient to dilute culture to the appropriate density (refer to the cell specific product insert). Adding fresh medium is sufficient to replenish cell nutrients. CO 2 exchange is not recommended for insect cell culture.